商品编号


AG-40T-0101-C050



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

50 ?g


商品信息


More Information



Product Details



Synonyms

TPH2; TRPO; Tryptophan 2,3-dioxygenase; Tryptophan Oxygenase; Tryptophan Pyrrolase; Tryptophanase



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Full-length TDO (
Anopheles gambiae
) is fused at the N-terminus to a His-tag.



Application

Can be used to test potential kynurenine pathway inhibitors in the mosquito, by continuously monitoring the rate of NFK formation at 321nm (ε=3152/M cm) in
in vitro
spectrophotometric assays.



BIOLOG
ical Activity

Specific activity towards L-Trp: 87.0μmol/hr per mg. TDO catalyzes the oxidative opening of the L-tryptophan indole moiety to yield N-formylkynurenine (NFK).



MW

~47.9kDa



Purity

≥98% (SDS-PAGE)



Endotoxin Content

N/A.



Concentration

0.5mg/ml (Lot specific)



Formulation

Liquid. In 50mM sodium phosphate pH 8.0, containing 0.15M sodium chloride, 0.2M imidazole and 10% glycerol.



Other Product Data

UniProt link O77457: TDO (
Anopheles gambiae
)



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

After opening, prepare aliquots and store at -80°C.
Avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 6 months after receipt when stored at -80°C.



Documents



MSD
S


Download PDF



Product Specification Sheet



Datasheet


Download PDF





Tryptophan 2,3-dioxygenase (TDO) is a heme-containing dioxygenase catalyzing the addition of molecular oxygen across the 2,3-double bond of the indole ring of tryptophan to form N-formylkynurenine (NFK). In
Anopheles gambiae
, TDO is the only enzyme able to catalyze the first and rate-limiting step in L-Trp catabolism through the kynurenine pathway. Tryptophan is an essential amino acid for protein synthesis and also the precursor for production of a number of neurotransmitters, such as
SERO
tonin and melatonin; in mosquitoes, the kynurenine pathway is essential for eye pigmentation. Conceivably, the tryptophan-using pathways should be regulated in a coordinated manner in mosquitoes as well as in other species and TDO activation/inactivation processes could play an essential role in these phenomena.


Product References

Anopheles gambiae tryptophan 2,3-dioxygenase expressed in Escherichia coli: A. Paglino, et al.; Insect Biochem. Mol. Biol.
38
, 871 (2008)