商品编号


AG-40T-0391-R100



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

100 ?l

商品信息


More Information



Product Details



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Four human ubiquitin L73P mutants (Accession Nr. P0CG47) covalently linked through isopeptide bonds at K48 residues of one ubiquitin molecule and the C-terminal glycine residue of another ubiquitin molecule, and coupled to agarose.



Crossreactivity

Human



Label/Conjugates

Agarose



MW

~34kDa



Formulation

Liquid. In 20% Ethanol.



Other Product Data


Use:
Linkage-specific, non-hydrolyzable tetra-ubiquitin is resistant to the activity of deubiquitinating enzymes (DUB's) that cleave the isopeptide linkage between adjacent ubiquitin molecules. May be useful for investigating ubiquitin-binding proteins and exploring the role of unanchored (free C-terminus) ubiquitin chains in signaling pathways. Tetra-ubiquitin is the minimal unit necessary for recognition by the 26S Proteasome and contains structural characteristics (such as repeating hydrophobic patches) not present in di-ubiquitin. Useful for the enrichment of known ubiquitin chain-interacting proteins as well as the discovery of novel ubiquitin chain-interacting proteins. We recommend equilibrating the resin by washing with 10 volumes of your desired aqueous buffer.

Storage:
Polyubiquitin-agarose can be re-used if properly maintained. After use, clean resin with a wash cycle of 5 volumes 100 mM HEPES pH 8.0, 500 mM NaCl followed by 5 volumes 100 mM NaOAc pH 4.5, 500 mM NaCl. Repeat twice, then rinse resin with a low salt buffer. Store resin at 4°C in neutral aqueous buffer containing 1 mM NaN3 or 20 % ethanol as a preservative.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

+4°C



Handling Advice

Do not freeze.



Use/St
ABI
lity

Stable for at least 3 months after receipt when stored at +4°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





With a predicted molecular weight of 34 kDa, Tetra-ubiquitin chains are composed of four ubiquitin monomers that are covalently linked through isopeptide bonds, which typically form between a lysine residue of one ubiquitin molecule and the C-terminal glycine residue of another ubiquitin molecule. Each human ubiquitin monomer is 76 amino acids (aa) in length and shares 96% and 100% aa identity with yeast and mouse ubiquitin, respectively. Seven of the 76 aa in ubiquitin are lysine residues that can participate in poly-ubiquitin chain formation. Linkage through specific lysine residues is thought to serve as a signal that affects protein degradation, signaling, trafficking, and other cellular processes.