商品编号


AG-40T-0397B-C025



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

25 ?g

商品信息


More Information



Product Details



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Four human ubiquitin (Accession Nr. P0CG47) covalently linked through isopeptide bonds at K48 residues of one ubiquitin molecule and the C-terminal glycine residue of another ubiquitin molecule and conjugated to biotin.



Crossreactivity

Human



Label/Conjugates

Biotin



Formulation

Lyophilized from a solution in deionized water.



Other Product Data


Use:
Formed with wild-type human recombinant ubiquitin and linkage-specific enzymes. Modified with biotin via primary amine coupling. This results in multiple biotinylated species modified at the N-terminus, as well as lysine residues. Biotinylated ubiquitin can be detected using avidin-linked reagents. Linkage-specific tetra-ubiquitin can be used to investigate mechanism of binding and recognition by E1 or E2 enzymes, deubiquitinating enzymes, E3 ligases or other proteins that contain ubiquitin-associated domains or ubiquitin-interacting motifs (UIMs). Tetra-ubiquitin is the minimal unit necessary for recognition by the 26S proteasome and contains structural characteristic (such as repeating hydrophobic patches) not present in di-ubiquitin. Ideal for use in assays that utilize avidin-linked reagents for visualization or quantitation. Reaction conditions will need to be optimized for each specific application.
IMPORTANT:
Heating this product in SDS-PAGE buffer or terminating reactions containing this product with heated SDS-PAGE buffer could lead to unexpected, high apparent molecular weight banding or smearing on gels that is not representative of product purity. For optimal results, we recommend incubation in SDS-PAGE buffer + DTT at <40°c for="" 20="" minutes="" prior="" to="" gel="" electrophoresis.="">40°c>



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





With a predicted molecular weight of 34 kDa, Tetra-ubiquitin chains are composed of four ubiquitin monomers that are covalently linked through isopeptide bonds, which typically form between a lysine residue of one ubiquitin molecule and the C-terminal glycine residue of another ubiquitin molecule. Each human ubiquitin monomer is 76 amino acids (aa) in length and shares 96% and 100% aa identity with yeast and mouse ubiquitin, respectively. Seven of the 76 aa in ubiquitin are lysine residues that can participate in poly-ubiquitin chain formation. Linkage through specific lysine residues is thought to serve as a signal that affects protein degradation, signaling, trafficking and other cellular processes.