商品编号


CHI-HF-210TGFBM-C100



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

100 ?g

商品信息


More Information



Product Details



Synonyms

Transforming Growth Factor beta-1; TGFB1; TGFB



Product Type

Protein



Properties



Source/Host

CHO cells



Sequence

The extracellular domain of a mutant human TGFβ1 is fused to the N-terminus of the Fc region of human IgG4. Site-directed mutagenesis was used to change three cysteine codons into a serine codon that are located in the pro region of the TGF-β precursor at amino acid positions 33, 223, and 225.



Crossreactivity

Human
Mouse



Specificity

Binds human TGFβ. Shown to cross-react with mouse TGFβ (see lit. reference 1).



BIOLOG
ical Activity

Shows the
BIOLOG
ical function of the TGFβ1 moiety and exerts a prolonged circulating half-life caused by the modified Fc domain.



Purity

≥98% (SDS-PAGE)



Endotoxin Content

<0.06eu mu;g="" protein="" (lal="" test;="" lonza).="">0.06eu>



Reconstitution

Reconstitute with 100 ?l sterile water.
Add 1X PBS to the desired protein concentration.



Formulation

Lyophilized from 0.2μm-filtered solution in PBS.



Declaration

Manufactured by Chimerigen.



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

Avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





TGF-β1 (Transforming growth factor beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. In humans, TGF-β1 is encoded by the TGFB1 gene. TGF-β1 was first identified in human platelets as a protein with a molecular mass of 25 kaD with a potential role in wound healing. It was later characterized as a large protein precursor (containing 390 aa) that was proteolytically processed to produce a mature peptide of 112 aa. TGF-β1 plays an important role in controlling the immune system, and shows different activities on different types of cell, or cells at different developmental stages. Most immune cells (or leukocytes) secrete TGF-β1.


Product References

Combined admi
NIST
ration of a mutant TGF-beta1/Fc and rapamycin promotes induction of regulatory T cells and islet allograft tolerance: W. Zhang, et al.; J. Immunol.
185,
4750 (2010)