商品编号


CHI-MF-110T3-C100



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

100 ?g

商品信息


More Information



Product Details



Synonyms

TIM3; TIMD3; Hepatitis A Virus Cellular Receptor 2; HAVcr-2; T Cell Immunoglobulin and Mucin Domain-containing Protein 3



Product Type

Protein



Properties



Source/Host

HEK 293 cells



Sequence

The extracellular domain of mouse Tim-3 (aa 21-189) is fused to the N-terminus of the Fc region of mouse IgG2a.



Crossreactivity

Mouse



BIOLOG
ical Activity

Immobilized recombinant human Galectin-9 at 500ng/ml can bind Tim-3 (mouse):Fc (human) (rec.) with an apparent KD <10nm. optimal="" dilutions="" should="" be="" determined="" by="" each="" laboratory="" for="" each="" application.="">10nm.>



MW

~65kDa (SDS-PAGE)



Purity

≥98% (SDS-PAGE)



Endotoxin Content

<5eu g="" protein="" (lal="" test;="" lonza).="">5eu>



Reconstitution

Reconstitute with 100 ?l sterile water. Add 1X PBS to the desired protein concentration.



Formulation

Lyophilized from 0.2μm-filtered solution in PBS.



Other Product Data

NCBI reference AAI06852.1: Tim-3 (mouse)



Declaration

Manufactured by Chimerigen.



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

Avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





The TIM (T cell/transmembrane, immunoglobulin and mucin) family plays a critical role in regulating immune responses, including allergy, asthma, transplant tolerance, autoimmunity and the response to viral infections. The unique structure of TIM immunoglobulin variable region domains allows highly specific recognition of phosphatidylserine (PtdSer), exposed on the surface of apoptotic cells. Tim-3, a type I transmembrane protein, contains an immunoglobulin and a mucin-like domain in its extracellular portion and a tyrosine phosphorylation motif in its cytoplasmic portion. TIM-3 is preferentially expressed on Th1 and Tc1 cells, and generates an inhibitory signal resulting in apoptosis of Th1 and Tc1 cells. TIM-3 is also expressed on some dendritic cells and can mediate phagocytosis of apoptotic cells and cross-presentation of antigen. Tim-3 functions to inhibit aggressive Th1-mediated auto- and alloimmune responses. Tim-3 pathway blockade by admi
NIST
ration of Tim-3:Fc fusion protein accelerates diabetes in nonobese diabetic mice, causes hyperproliferation of Th1 cells and Th1 cytokine release in an experimental autoimmune encephalomyelitis (EAE) model and prevents acquisition of transplantation tolerance induced by costimulation blockade.


Product References

Interaction of Tim-3 and Tim-3 ligand regulates T helper type 1 responses and induction of peripheral tolerance: C.A. Sabatos, et al.; Nat. Immunol.
4,
1102 (2003)

The Tim-3 ligand galectin-9 negatively regulates T helper type 1 immunity: C. Zhu, et al.; Nat. Immunol.
6,
1245 (2005)

TIM-3 is expressed on activated human CD4+ T cells and regulates Th1 and Th17 cytokines: W.D. Hastings, et al.; Eur. J. Immunol.
39,
2492 (2009)