商品编号


AG-40T-0019-C002



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

2 ?g


商品信息


More Information



Product Details



Synonyms

Histone Acetyltransferase HTATIP; Histone Acetyltransferase KAT5; Lysine Acetyltransferase 5; 60 kDa Tat-interactive Protein



Product Type

Protein



Properties



Source/Host

Sf21 cells



Sequence

Human TIP60 is fused to a His-tag.



Crossreactivity

Human



BIOLOG
ical Activity

100-200ng are required for standard HAT assay.



Purity

≥95% (SDS-PAGE)



Concentration

Lot dependent (0.2-0.5mg/ml)



Formulation

Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 0.2% NP-40, 50mM imidazole and 10% glycerol.



Other Product Data

UniProt ID Q92993: Tip60 (human)



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

After opening, prepare aliquots and store at -80°C.
Avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 6 months after receipt when stored at -80°C.



Documents



MSD
S


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Product Specification Sheet



Datasheet


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TIP60 is the catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex is required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. TIP60 directly acetylates and activates ataxia-telangiectasia mutated (ATM). In case of HIV-1 infection, interaction with the viral Tat protein leads to KAT5 polyubiquitination and targets it to degradation.


Product References

Functional relevance of novel p300-mediated lysine 314 and 315 acetylation of RelA/p65: C. Buerki, et al.; Nucleic Acids Res.
36,
1665 (2008)

Oridonin, a novel lysine acetyltransferases inhibitor, inhibits proliferation and induces apoptosis in gastric cancer cells through p53- and caspase-3-mediated mechanisms: M. Shi, et al.; Oncotarget
7,
22623 (2016)