商品编号


AG-40B-0019-C010



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

10 ?g


商品信息


More Information



Product Details



Synonyms

MultimericTNF-α?; ACRP30
headless
:TNF-α; ACRP30
headless
:TNFSF2; ACRP30
headless
:Tumor Necrosis Factor-α



Product Type

Protein



Properties



Source/Host

HEK 293 cells



Sequence

The extracellular domain of human TNF-α (aa 85-233) is fused at the N-terminus to mouse ACRP30
headless
(aa 18-111) and a FLAG
?
-tag.



Crossreactivity

Human
Mouse



Specificity

Binds to human and mouse TNF-R1 and TNF-R2.



BIOLOG
ical Activity

Activates human and mouse TNF-R1 and TNF-R2. Induces cell death at a concentration range of 0.01-10ng/ml (ED
50
= 0.05ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells).



MW

~34kDa (SDS-PAGE)



Purity

≥95% (SDS-PAGE)



Endotoxin Content

<0.02EU/μg purified protein (LAL test;
Lonza
).



Concentration

0.1mg/ml after reconstitution.



Reconstitution

Reconstitute with 100μl sterile water.



Formulation

Lyophilized. Contains PBS.



Other Product Data


UniProt link P01375: TNF-α (human)

FLAG is a registered trademark of
Sigma-Aldrich
Co.




Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.



Use/St
ABI
lity

Stable for at least 6 months after receipt when stored at -20°C.



Documents



MSD
S


Download PDF



Product Specification Sheet



Datasheet


Download PDF





Tumor necrosis factor (TNF, cachexin or cachectin and formerly known as tumor necrosis factor-α) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction.
Multimeric
TNF-α? is a high activity construct in which two trimeric TNF-α ligands are artificially linked via the collagen domain of ACRP30. The receptor TNF-R1 is activated by both the membrane-bound and soluble trimeric forms of TNF-α, whereas the receptor TNF-R2 only responds to the membrane-bound form of TNF-α (TNF-α (human) (multimeric) (rec.) (Prod. No. AG-40B-0019). Since the
Multimeric
TNF-α? mimics the membrane-bound form (forms oligomers higher than trimer), it is the only TNF-α protein that can activate the TNF-R2. For TNF-R1 activation, either "normal" TNF-α or
Multimeric
TNF-α? can be used.