商品编号


CHI-HR-200TNF-C010



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

10 ?g

商品信息


More Information



Product Details



Synonyms

Tumor Necrosis Factor Ligand Superfamily Member 2; Cachectin



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human TNF-α (aa 77-233) is fused at the C-terminus to a His-tag.



Crossreactivity

Human
Mouse



BIOLOG
ical Activity

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.



MW

~18kDa (SDS-PAGE)



Purity

≥95% (SDS-PAGE)



Endotoxin Content

<0.1eu g="" purified="" protein="" (lal="" test;="" lonza).="">0.1eu>



Reconstitution

Reconstitute 10?g vial with 100 ?l sterile water to a concentration of 0.1mg/ml.
Reconstitute 50?g vial with 100 ?l sterile water to a concentration of 0.5mg/ml.
Add 1X PBS to the desired protein concentration.



Formulation

Lyophilized from 0.2μm-filtered solution in 10mM sodium citrate buffer, 200mM NaCl, pH6.0.



Other Product Data

NCBI reference AAH28148.1: TNF-alpha (human)



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells, including
ADI
pocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands, and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells, and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation, and diabetes. Recombinant human TNF-α is a soluble 157 amino acid protein (17.4 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.