商品编号


AG-40B-0006-C010



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

10 ?g


商品信息


More Information



Product Details



Synonyms

Tumor Necrosis Factor Ligand Superfamily Member 2; Cachectin



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

A FLAG
?
-tag is fused at the N-terminus of human TNF-α (aa 85-223).



Crossreactivity

Human
Mouse



Specificity

Binds to human and mouse TNF-R1.



BIOLOG
ical Activity

Induces cell death at a concentration range of 0.01-10ng/ml (ED
50
= 0.05ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells).



MW

~17kDa (SDS-PAGE)



Purity

≥95% (SDS-PAGE)



Endotoxin Content

<0.01eu g="" purified="" protein="" (lal="" test;="" lonza).="">0.01eu>



Concentration

10 ?g size: 0.1 mg/ml after reconstitution.
50 ?g size: 1 mg/ml after reconstitution.




Reconstitution


10 ?g size:
Reconstitute with 100 ?l sterile water.
50 ?g size:
Reconstitute with 50 ?l sterile water.
PBS containing at least 0.1% BSA should be used for further dilutions.



Formulation

Lyophilized. Contains PBS.



Other Product Data

UniProt link P01375: TNF-α (human) (precursor)

FLAG is a registered trademark of
Sigma-Aldrich
Co.




Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.



Documents



MSD
S


Download PDF



Product Specification Sheet



Datasheet


Download PDF





Tumor necrosis factor (TNF, cachexin or cachectin and formerly known as tumor necrosis factor-α) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. The receptor TNF-R1 is activated by both the membrane-bound and soluble trimeric forms of TNF-α, whereas the receptor TNF-R2 only responds to the membrane-bound form of TNF-α (
Multimeric
TNF-α (Prod. No. AG-40B-0019). Since the
Multimeric
TNF-α mimics the membrane-bound form (forms oligomers higher than trimer), it is the only TNF-α protein that can activate the TNF-R2. For TNF-R1 activation, either "normal" TNF-α or
Multimeric
TNF-α can be used.


Product References

CXCL-8/IL8 Produced by Diffuse Large B-cell Lymphomas Recruits Neutrophils Expressing a Proliferation-Inducing Ligand APRIL: B. Manfroi, et al.; Cancer Res.
77,
1097 (2017)