商品编号


AG-40T-0410B-C025



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

25 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin-conjugating Enzyme E2; E2-25K; Hip2; HYPG; LIG; UBE2K; UBE2K-25K; Huntingtin-interacting Protein 2



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human UbcH1 (Accession Nr. P61086) conjugated to biotin.



Crossreactivity

Human



Label/Conjugates

Biotin



Formulation

Liquid.



Other Product Data


Use:
Can be used as a positive control for sumoylation assays. It is modified by SUMO-1, SUMO-2 and SUMO-3 proteins and can be detected with avidin-linked reagents. Typical concentration to use in SUMO conjugation experiments
in vitro
is 1-5μM depending on conditions.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin-conjugating Enzyme E2K (UBE2K), also known as UbcH1, E2-25K, HIP2 and LIG, is a 200 amino acid (aa) protein with a predicted molecular weight of 25 kDa. Human and mouse UbcH1 share 100% aa sequence identity. Similar to other ubiquitin-conjugating (E2) enzymes, UbcH1 contains a conserved ubiquitin-conjugating domain but is unique in that it also has a C-terminal, non-covalent ubiquitin binding surface termed the ubiquitin-associated domain between aa residues 160 and 200. UbcH1 mediates the elongation of Lys48-linked poly-ubiquitin chains. UbcH1 catalytic activity can be modulated by the post-translational addition of SUMO at Lys14. Substrates include the Huntingtin protein and the tumor suppressor RB1. UbcH1 is widely expressed, with highest levels found in the brain cortex and striatum, and dysregulated UbcH1 is implicated in polyglutamine diseases and Alzheimer's disease.