商品编号


AG-40T-0411-C100



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

100 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin-conjugating Enzyme E2 C; Ubiquitin-protein Ligase C; UBE2C



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human UbcH10 (Accession Nr. O00762) fused to a N-terminal His-tag.



Crossreactivity

Human



Formulation

Liquid. In 50mM HEPES pH 8.0, 200mM NaCl, 10% Glycerol (v/v), 1mM TCEP.



Other Product Data


Use:
Member of the ubiquitin?conjugating (E2) enzyme family that receives ubiquitin from a ubiquitin?activating (E1) enzyme and subsequently interacts with a ubiquitin ligase (E3) to conjugate ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.1?-1μM.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin-conjugating Enzyme H10 (UbcH10), also known as ubiquitin-conjugating enzyme E2C (UBE2C), is a 179 amino acid (aa) member of the yeast Ubc4/5 family of ubiquitin-conjugating (E2) enzymes and has a predicted molecular weight of 20 kDa. Human UbcH10/UBE2C shares 96% aa sequence identity with mouse and rat UBE2C. UbcH10/UBE2C is an essential mediator of mitotic destruction events and cell cycle progression. UbcH10/UBE2C recognizes TEK sequences in target proteins such as Cyclins A and B, mediates Lys11-linked ubiquitination and promotes target protein degradation in conjunction with APC/C, a ubiquitin ligase (E3). The catalytic activity of UbcH10/UBE2C is regulated by a conserved N-terminal extension, which mediates E2-E3 interaction. UbcH10/UBE2C is overexpressed in a variety of human cancers and alternate splice isoforms may contribute to uncontrolled cell proliferation and tumor progression.