商品编号


AG-40T-0413-C100



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

100 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin-conjugating Enzyme E2 M; UBC12; UBC-RS2; NEDD8-conjugating Enzyme Ubc12; NEDD8 Protein Ligase; UBE2M



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human UbcH12 (Accession Nr. P61081) fused to a N-terminal GST-tag.



Crossreactivity

Human



Formulation

Liquid. In 50mM HEPES pH 7.0, 200mM NaCl, 10% Glycerol (v/v), 1mM TCEP.



Other Product Data


Use:
Member of the NEDD8-conjugating (E2) enzyme family that receives NEDD8 from a NEDD8-activating (E1) enzyme and subsequently interacts with a NEDD8 ligase (E3) to conjugate NEDD8 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.1?-1μM.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin-conjugating Enzyme E2M (UBE2M), also known as ubiquitin-conjugating enzyme 12 (Ubc12), is a ubiquitously expressed protein with a predicted molecular weight of 20 kDa. UBE2M/Ubc12 is highly conserved, and the human ortholog shares 100% amino acid identity with mouse and rat UBE2M. UBE2M/Ubc12 mediates conjugation of the ubiquitin-like protein neural precursor cell expressed developmentally down-regulated 8 (NEDD8) to substrate proteins. Neddylation requires the coordinated activities of UBE2M/Ubc12 and the APPBPI/UBA3 heterodimer, a NEDD8-activating (E1) enzyme. The NEDD8 pathway is essential for the vi
ABI
lity of many organisms and plays important roles in morphogenesis and cell cycle progression. UBE2M/Ubc12-dependent neddylation modulates the levels of p27/Kip1, β-Catenin, Cdc53/Cul1 and other members of the Cullin family.