商品编号


AG-40T-0416-C100



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

100 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin-conjugating Enzyme E2 H; E2-14K; E2-20K; UBC8; UBE2H; Ubiquitin-protein Ligase H



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human UbcH2 (Accession Nr. NP_003335) fused to a N-terminal GST-tag.



Crossreactivity

Human



Formulation

Liquid. In 50mM HEPES, pH 8.0, 100mM NaCl, and 10% Glycerol



Other Product Data


Use:
Member of the ubiquitin?conjugating (E2) enzyme family that receives ubiquitin from a ubiquitin?activating (E1) enzyme and subsequently interacts with a ubiquitin ligase (E3) to conjugate ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.1-?1μM.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin-conjugating Enzyme H2 (UbcH2), also known as ubiquitin-conjugating Enzyme E2H (UBE2H), is a member of the ubiquitin-conjugating (E2) enzyme family. UbcH2/UBE2H has a predicted molecular weight of 20.5 kDa. The human protein shares 100% amino acid sequence identity with the mouse and rat orthologs. Human UbcH2/UBE2H is highly expressed in skeletal and cardiac muscle, shows significant expression levels in kidney and placenta, and is expressed at low levels in liver, brain, lung, and spleen. TNF-α induces UbcH2/UBE2H expression via NF-κB, and SCL/Tal1 upregulates the expression of UbcH2/UBE2H during erythroid differentiation. Pathologically, a single nucleotide polymorphism within UbcH2/UBE2H is associated with autism. Fur
Thermo
re, UbcH2/UBE2H has been reported as a putative oncogene for liver tum
Origen
esis.