商品编号


AG-40T-0425-C100



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

100 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin-conjugating Enzyme E2 E1; Ubiquitin-protein Ligase E1; UBE2E1



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human UbcH6 (Accession Nr. P51965) fused to a N-terminal His-tag.



Crossreactivity

Human



Formulation

Liquid. In 50mM HEPES pH 7.5, 200mM NaCl, 10% Glycerol (v/v), 1mM TCEP.



Other Product Data


Use:
Member of the ubiquitin?conjugating (E2) enzyme family that receives ubiquitin from a ubiquitin?activating (E1) enzyme and subsequently interacts with a ubiquitin ligase (E3) to conjugate ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.1-?1μM.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin-conjugating Enzyme H6 (UbcH6), also known as ubiquitin-conjugating Enzyme E2E 1 (UBE2E1), is a ubiquitously expressed protein with a predicted molecular weight of 21 kDa. Human UbcH6/UBE2E1 shares 99% and 100% amino acid sequence identity with mouse and rat UBE2E1, respectively. UbcH6/UBE2E1 mediates the selective degradation of short-lived and abnormal proteins. UbcH6/UBE2E1 catalytic activity is modulated following the addition of the ubiquitin-like protein ISG15 to Lys136. In the nucleus, the N-terminal fragment of UbcH6/UBE2E1 interacts with the ubiquitin ligase (E3) TRIM21 to mediate substrate ubiquitination. UbcH6/UBE2E1 also interacts with the viral E3, ICP0, during HSV-1 infection. In addition, UbcH6/UBE2E1-dependent protein ubiquitination is implicated in polyglutamine tract disorders such as ataxia.