商品编号


AG-40T-0428-C100



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

100 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin-conjugating Enzyme H8; RIG-B; Ubiquitin/ISG15-conjugating Enzyme E2 L6; Ubiquitin-protein Ligase L6; UBE2L6



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human UbcH8 (Accession Nr. O14933) fused to a N-terminal GST-tag.



Crossreactivity

Human



Formulation

Liquid. In 50mM HEPES pH 7.5, 100mM NaCl, 10% Glycerol (v/v), 1mM TCEP.



Other Product Data


Use:
Member of the ubiquitin and ISG15?conjugating (E2) enzyme family that receives ubiquitin and ISG15 from a ubiquitin and ISG15?activating (E1) enzyme and subsequently interacts with a ubiquitin and ISG15 ligase (E3) to conjugate ubiquitin and ISG15 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.1-?1μM.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin-conjugating Enzyme H8 (UbcH8), also known as ubiquitin-conjugating Enzyme E2L 6 (UBE2L6), has a predicted molecular weight of 18 kDa. Human UbcH8 shares 76% and 74% amino acid sequence identity with mouse and rat UBE2L6, respectively. UbcH8 is capable of transferring either ubiquitin or the ubiquitin-like protein ISG15 ubiquitin-like modifier (ISG15) to target proteins. ISG15 conjugation is mediated by UbcH8 following IFN-α or IFN-β stimulation. UbcH8 interacts with a variety of ubiquitin ligases (E3s) to transfer ubiquitin to target proteins such as the cell cycle regulator, p21/CIP1/CDKN1A. UbcH8 also regulates actin
Cytoskeleton
dynamics, and dysregulated UbcH8 promotes migration and invasion of human breast cancer cells. In addition, UbcH8 interacts with the E3, Parkin, and is thought to play a role in the pathogenesis of Parkinson’s disease.