商品编号


AG-40T-0437-C100



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

100 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin-conjugating Enzyme E2 G2; UBC7; Ubiquitin-protein Ligase G2



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human UBE2G2 (Accession Nr. NP_003334) fused to a N-terminal His-tag.



Crossreactivity

Human



Formulation

Liquid. In 50mM HEPES pH 7.5, 100mM NaCl, 10% Glycerol (v/v), 1mM TCEP.



Other Product Data


Use:
Member of the ubiquitin?conjugating (E2) enzyme family that receives ubiquitin from a ubiquitin?activating (E1) enzyme and subsequently interacts with a ubiquitin ligase (E3) to conjugate ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.1-?1μM.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin-conjugating Enzyme E2G 2 (UBE2G2), also known as ubiquitin-conjugating enzyme 7 (Ubc7), is a ubiquitously expressed member of the ubiquitin-conjugating (E2) enzyme family that localizes to both the nucleus and cytoplasm. UBE2G2 has a predicted molecular weight of 18 kDa. The human protein shares 100% amino acid sequence identity with the mouse and rat orthologs. UBE2G2 interacts with the HRD1 and gp78 ubiquitin ligases (E3s) to mediate efficient endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins in human cells. Mecha
NIST
ically, it has been shown that UBE2G2 forms Lys48-linked ubiquitin chains on itself prior to gp78-dependent transfer of the ubiquitin chains to target proteins. UBE2G2 also interacts with the CRL4(Cdt2) E3 complex to promote the ubiquitination and degradation of Cdt1 following UV irr
ADI
ation, suggesting that UBE2G2 may function in the response to DNA damage.