商品编号


AG-40T-0438-C100



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

100 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin-conjugating Enzyme E2 J1; CGI-76; HSPC153; NCUBE1; UBC6e; Non-canonical Ubiquitin-conjugating Enzyme 1; Yeast Ubiquitin-conjugating Enzyme UBC6 Homolog E



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human UBE2J1 (Accession Nr. Q9Y385) fused to a N-terminal His-tag.



Crossreactivity

Human



Formulation

Liquid. In 50mM HEPES pH 8.0, 200mM NaCl, 10% Glycerol (v/v), 1mM TCEP.



Other Product Data


Use:
Member of the ubiquitin?conjugating (E2) enzyme family that receives ubiquitin from a ubiquitin?activating (E1) enzyme and subsequently interacts with a ubiquitin ligase (E3) to conjugate ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.1?-1μM.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


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Ubiquitin-conjugating Enzyme E2, J1 (UBE2J1), also known as HsUbc6e, is a 318 amino acid (aa) member of the ubiquitin-conjugating (E2) enzyme family with a predicted molecular weight of approximately 35 kDa. The human protein shares 92% and 85% aa sequence identity with the mouse and rat orthologs, respectively. UBE2J1 has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with ubiquitin. It localizes to endoplasmic reticulum (ER) membranes where it participates in ER-associated degradation (ERAD) of misfolded proteins. In response to ER stress, UBE2J1 is phosphorylated on Ser184 in a PERK-dependent manner. It has also been shown to function with the HRD1 ubiquitin ligase (E3) to promote the ubiquitination and degradation of misfolded MHC class I heavy chain via ERAD. Additionally, UBE2J1 may have a preventative role in early-onset systemic amyloidosis. It has a redundant role, along with the UBE2G2 E2 enzyme, in promoting the degradation of a mutant form of transthyretin that is associated with this disease.