商品编号


AG-40T-0439-C100



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

100 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin-conjugating Enzyme E2 J2; NCUBE2; PRO2121; Ubc6; Ubc6p; Non-canonical Ubiquitin-conjugating Enzyme 2



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human UBE2J2 Isoform 1 (Accession Nr. Q8N2K1) fused to a N-terminal His-tag.



Crossreactivity

Human



Formulation

Liquid. In 50mM HEPES pH 7.5, 200mM NaCl, 10% Glycerol (v/v), 1mM TCEP.



Other Product Data


Use:
Member of the ubiquitin?conjugating (E2) enzyme family that receives ubiquitin from a ubiquitin?activating (E1) enzyme and subsequently interacts with a ubiquitin ligase (E3) to conjugate ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.1?-1μM.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin-conjugating Enzyme E2, J2 (UBE2J2) is a 259 amino acid (aa) member of the ubiquitin-conjugating (E2) enzyme family with a predicted molecular weight of approximately 29 kDa. The human protein shares 100% aa sequence identity with the mouse and rat orthologs. UBE2J2 has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with ubiquitin. This enzyme has been reported to be involved in the ER-associated degradation pathway. UBE2J2 has also been shown to interact with the mK3 ubiquitin ligase (E3) and promote the ubiquitination of hydroxylated residues on major histocompatibility complex class I heavy chains.