商品编号


SBB-CE0022-C100



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

100 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin-conjugating Enzyme E2; E2-25K; Hip2; HYPG; LIG; UBE2K; UBE2K-25K; Huntingtin-interacting Protein 2



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human UBE2K (aa 1-200) (Accession Nr. P61086) is fused at the N-terminus to a His-tag.



Crossreactivity

Human



Application

Typical working concentration range is 1-5?M.



MW

~22kDa (SDS-PAGE)



Purity

≥95% (SDS-PAGE)



Concentration

Lot dependent.



Accession Number

P61086



Formulation

Liquid. In 50mM HEPES pH 7.5, 150mM sodium chloride, 10% glycerol (v/v), 2mM TCEP.



Other Product Data

Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer
Our product description may differ slightly from the original manufacturers product datasheet.



Declaration

Manufactured by South Bay Bio.



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-80°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





UBE2K (UbcH1) is an E2 ubiquitin conjugating enzyme. An E1 activating enzyme is required to attach ubiquitin to UBE2K via an active site cysteine. The mechanism of ubiquitin transfer involves the breaking of a E1-Ub thioester linkage, followed by a reformation of a UBE2K-Ub thioester. UBE2K is capable of synthesizing K48-linked ubiquitin chains and can do so without an E3 ubiquitin ligating enzyme present. UBE2K catalyzes free K48-linked polyubiquitin chains and is known to interact with huntingtin and TRIM6. Although an E3 ligase is not required for chain formation, free UBE2K synthesized K48 polyubiquitin chains have been shown to interact with the E3 TRIM6, le
ADI
ng to activation of IKKε kinase activity and subsequent antiviral activity.