商品编号


AG-40T-0442-C100



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

100 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin-conjugating Enzyme E2 Q2; Ubiquitin-protein Ligase Q2



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human UBE2Q2 Isoform 1 (Accession Nr. Q8WVN8-1) fused to a N-terminal His-tag.



Crossreactivity

Human



Formulation

Liquid. In 50mM HEPES, pH 7.5, 200mM NaCl, 10% Glycerol (v/v), 2mM DTT.



Other Product Data


Use:
Member of the ubiquitin?conjugating (E2) enzyme family that receives ubiquitin from a ubiquitin?activating (E1) enzyme and subsequently interacts with a ubiquitin ligase (E3) to conjugate ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.1-?1μM.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin-conjugating Enzyme E2Q Family Member 2 (UBE2Q2) is a 375 amino acid (aa) member of the ubiquitin-conjugating (E2) enzyme family with a predicted molecular weight of approximately 43 kDa. Human UBE2Q2 shares 95% aa sequence identity with its mouse ortholog. UBE2Q2 contains an RWD domain, a coiled-coil domain and an E2 catalytic core domain. The E2 cataylic core domain contains an active site cysteine residue that is required for the formation of a thioester bond with ubiquitin. UBE2Q2 expression has been observed in both the cytoplasm and the nucleus. Expression of dominant-negative UBE2Q2 can cause a prolonged prophase arrest and UBE2Q2 overexpression inhibits cell proliferation, suggesting that UBE2Q2 may be involved in normal cell cycle progression. Elevated expression of UBE2Q2 has been reported in hypopharyngeal cancer, breast cancer and acute lymphoblastic leukemia, suggesting that this enzyme may function as an oncogene.