商品编号


AG-40T-0448-C100



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

100 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin-conjugating Enzyme E2 W; Ubc16; N-terminus-conjugating E2; Ubiquitin-protein Ligase W



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human UBE2W Isoform 2 (Accession Nr. Q96B02-2) fused to a N-terminal His-tag.



Crossreactivity

Human



Formulation

Liquid. In 50mM HEPES pH 7.5, 200mM NaCl, 10% Glycerol (v/v), 1mM TCEP.



Other Product Data


Use:
Member of the ubiquitin?conjugating (E2) enzyme family that receives ubiquitin from a ubiquitin?activating (E1) enzyme and subsequently interacts with a ubiquitin ligase (E3) to conjugate ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.1?-1μM.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin-conjugating Enzyme E2W (UBE2W), also known as ubiquitin-conjugating enzyme 16 (UBC16), is a member of the ubiquitin-conjugating (E2) enzyme family. It has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with ubiquitin. There are two isoforms of UBE2W, isoform 1 and isoform 2, with predicted molecular weights of 18.1 kDa and 19.5 kDa, respectively. UBE2W localizes to the nucleus where it promotes the mono-ubiquitination of BRCA1 and FANCD2, both of which contribute to DNA repair pathways. Mono-ubiquitination of the CHIP ubiquitin ligase (E3), which has been reported to enhance its E3 ligase activity, is also mediated by UBE2W.