商品编号


AG-40T-0449-C100



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

100 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin-conjugating Enzyme E2 Z; Uba6-specific E2 Conjugating Enzyme 1; Ubiquitin-protein Ligase Z; Use1



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human UBE2Z (Accession Nr. Q9H832) fused to a N-terminal His-tag.



Crossreactivity

Human



Formulation

Liquid. In 50mM HEPES pH 7.5, 200mM NaCl, 10% Glycerol (v/v), 1mM TCEP.



Other Product Data


Use:
Member of the ubiquitin?conjugating (E2) enzyme family that receives ubiquitin from a ubiquitin?activating (E1) enzyme and subsequently interacts with a ubiquitin ligase (E3) to conjugate ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.1-?1μM.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Uba6-specific E2 Conjugating Enzyme 1 (Use1), also known as ubiquitin-conjugating enzyme E2Z (UBE2Z), is a member of the ubiquitin-conjugating (E2) enzyme family that is highly expressed in human placenta, pancreas, spleen and testis. Use1/UBE2Z has a predicted molecular weight of 38 kDa. The human enzyme shares 98% amino acid sequence identity with its mouse and rat orthologs. It has an E2 catalytic core domain that contains an active site cysteine residue that is required for the formation of a thioester bond with ubiquitin. Use1/UBE2Z functions specifically with the UBE1L2/UBA6 ubiquitin-activating (E1) enzyme, rather than the UBE1 E1 enzyme, for ubiquitin conjugation. Use1/UBE2Z expression is enriched in the cytoplasm where it mediates the ubiquitination of RGS4 and RGS5 via the N-end rule proteolytic pathway. Use1/UBE2Z also functions with UBE1L2/UBA6 to mediate the conjugation of FAT10, a ubiquitin-like protein, and is auto-FAT10ylated in response to TNF-α and IFN-γ.