商品编号


AG-40T-0450-C050



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

50 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin-protein Ligase E3A; E6AP; Human Papillomavirus E6-associated Protein; ANCR; EPVE6AP; HPVE6A; Oncogenic Protein-associated Protein E6-AP; Renal Carcinoma Antigen NY-REN-54



Product Type

Protein



Properties



Source/Host

Sf21 cells



Sequence

Human UBE3A (Accession Nr. Q05086) fused to a C-terminal His-tag.



Crossreactivity

Human



Formulation

Liquid. In 50mM HEPES pH 8.0, 100mM NaCl, 1mM TCEP.



Other Product Data


Use:
Typical enzyme concentration to support conjugation
in vitro
is 1-5μM depending on experimental conditions.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





UBE3A, also called E6AP (E6-Associated Protein) is a HECT domain ubiquitin E3 ligase that ubiquitinates and mediates the proteasomal destruction of substrate proteins. HECT domain ligases use an active site cysteine to accept charged ubiquitin from ubiquitin-E2 thioester complexes for subsequent transfer to substrate proteins. In this way HECT class ligases are distinct from most RING class ligases in that the latter facilitate transfer of ubiquitin from charged E2’s directly to substrate proteins without an E3-ubiquitin thioester intermediate. The viral HPV-E6 protein (present in cells infected with human papillomavirus) forms a complex with E6AP to generate a ligase activity that polyubiquitinates the tumor suppressors p53 and p73 and targets them to the 26S proteasome for degradation.