商品编号


AG-40T-0453-C250



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

250 ?g

商品信息


More Information



Product Details



Synonyms

Protein Linking IAP with
Cytoskeleton
1; DA41; DSK2; PLIC-1; UBQLN1; UBQN; XDRP1



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human ubiquilin 1 Tandem UBA [TUBE2] (Accession Nr. Q9UMX0) fused to a His-tag.



Crossreactivity

Human



Formulation

Liquid. In 50mM HEPES, 200mM NaCl, 1mM DTT pH 8.0.



Other Product Data


Use:
Can be used for the isolation and identification of K48-linked (preferentially) or K63-linked poly-ubiquitin chains or ubiquitinated substrates that contain these linkages. Allows for metal chelate affinity purification and also allows for convenient immuno-detection of conjugates using His-specific antibodies. Use 50-100?g of protein to detect 2-25?g of purified K48-linked ubiquitin chains. The amount necessary for use in crude lysates needs to be determined empirically.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


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Ubiquilin 1 is a ubiquitously expressed member of the UBL-UBA family of ubiquitin-binding proteins that is 589 amino acids (aa) in length and has a predicted molecular weight of 62.5 kDa. Human ubiquilin 1 shares 88% aa sequence identity with the mouse and rat orthologs. It contains a N-terminal ubiquitin-like (UBL) domain and a C-terminal ubiquitin-associated (UBA) domain. The UBA domain is able to interact with mono- and poly-ubiquitin chains, with a similar affinity for Lys48- and Lys63-linked chains. In contrast, the UBL domain interacts with ubiquitin-interacting motif (UIM)-containing proteins, such as S5a/Angiocidin, a 19S Proteasome component. It is thought that ubiquilin 1 may act as a molecular shuttle, bringing poly-ubiquitinated proteins to the 26S Proteasome by simultaneously binding poly-ubiquitin via its UBA domain and the 19S Proteasome via its UBL domain. Ubiquilin 1 also binds two endoplasmic reticulum (ER)-localized proteins, Herp and Erasin, and may function in the ER-associated degradation pathway. It has been reported to co-precipitate with LC3/MAP1LC3A/Apg8p3, an autophagosomal
Marker
, suggesting that it may play a role in autophagy. Single nucleotide polymorphisms in the UBQLN1 gene have been linked to Alzheimer's disease (AD). Fur
Thermo
re, ubiquilin 1 may regulate the processing of APP, which is associated with AD. Tandem ubiquitin binding entities (TUBEs) have been developed for the isolation and identification of ubiquitinated proteins. TUBEs display increased affinity for poly-ubiquitin moieties over the single ubiquitin binding associated domain (UBA). TUBEs also display a protective effect on poly-ubiquitinated proteins, allowing for detection at relatively low abundance.