商品编号


AG-40T-0459F-C100



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

100 ?g

商品信息


More Information



Product Details



Synonyms

UBB; Ubiquitin B



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human ubiquitin (Accession Nr. P0CG47) conjugated to N-terminal FITC (multiple modifications).



Crossreactivity

Human



Label/Conjugates

FITC



Formulation

Lyophilized.



Other Product Data


Use:
Modified with fluorescein via primary amine coupling. This results in multiple fluoresceinated ubiquitin species with modified lysines as well as the N-terminus. Although having a fully functional C-terminus, lysine modification may limit the
ABI
lity of this reagent to efficiently incorporate into poly-ubiquitin chains. Gives a strong signal in the range of 0.1-1?M, depending on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored using Ex490nM and Em515nM wavelengths respectively.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast ubiquitin, and 100% aa sequence identity shared between human and mouse ubiquitin. In mammals, four ubiquitin genes encode for two ubiquitin-ribosomal fusion proteins and two poly-ubiquitin proteins. Cleavage of the ubiquitin precursors by deubiquitinating enzymes gives rise to identical ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a ubiquitin-activating (E1) enzyme, a ubiquitin-conjugating (E2) enzyme, and a ubiquitin ligase (E3). ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1). In addition to protein degradation, ubiquitination has been shown to mediate a variety of
BIOLOG
ical processes such as signal transduction, endocytosis, and post-endocytic sorting.