商品编号


AG-40T-0540-C050



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

50 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin C-terminal Hydrolase 25; Deubiquitinating Enzyme 25;
USP
on Chromosome 21; Ubiquitin Thioesterase 25; Ubiquitin-specific-processing Protease 25



Product Type

Protein



Properties



Source/Host

Sf21 cells



Sequence

Human
USP
25 (Accession Nr. Q9UHP3) fused to a C-terminal His-tag.



Crossreactivity

Human



Formulation

Liquid. In 50mM HEPES pH 8.0, 200mM NaCl, 2mM DTT.



Other Product Data


Use:
Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.1-1?M.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin Specific Peptidase 25 (
USP
25) is a cytoplasmic protein that belongs to the peptidase C19 family of deubiquitinating enzymes. It has been shown to be expressed as three tissue-specific isoforms:
USP
25a,
USP
25b, and
USP
25m. The canonical isoform,
USP
25a, is 1055 amino acids (aa) in length and has a predicted molecular weight of 122.2 kDa. Human
USP
25a shares 94% aa sequence identity with the mouse and rat orthologs. It is widely expressed with the highest levels being observed in fetal brain and adult testis.
USP
25b and
USP
25m are 1087 aa and 1125 aa in length with predicted molecular weights of 125.8 and 130 kDa, respectively.
USP
25b is found in all tissues except heart and skeletal muscle, while
USP
25m is exclusively expressed in heart and skeletal muscle. The catalytic domain of
USP
25 is found at aa 166-654.
USP
25 also contains a ubiquitin-associated domain (aa 16-57), a SUMO-interacting motif (aa 77-102) and two ubiquitin-interacting motifs (aa 97-116 and 123-140), which appear to be required for
in vitro
hydrolysis of Lys48- and Lys63-linked poly-ubiquitin chains.
USP
25 has been shown to interact with the non-receptor tyrosine kinase SYK, which phosphorylates
USP
25 on Tyr740, le
ADI
ng to a decrease in its intracellular levels.
USP
25 can also be SUMOylated on Lys99 and Lys141. This inhibits
USP
25 activity by impeding its binding to poly-ubiquitin chains.
USP
25m can also be ubiquitinated on Lys99 and is thought to stimulate
USP
25m activity.
USP
25 has been shown to inhibit the degradation of endoplasmic reticulum proteins and to negatively regulate inflammatory responses induced by IL-17. Additionally,
USP
25 has been found to be overexpressed in Down Syndrome fetal brains.
USP
25m is thought to be involved in the regulation of muscular differentiation and function. Its expression is up-regulated during myogenesis, and it has been shown to interact with three sarcomeric proteins critical for muscle differentiation and maintenance, ACTA1, FLNC and MyBPC1.