商品编号


AG-40T-0542-C050



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

50 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin C-terminal Hydrolase 7; Deubiquitinating Enzyme 7 Herpesvirus-associated Ubiquitin-specific Protease; Ubiquitin Thioesterase 7; Ubiquitin Specific Protease 7; HA
USP




Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human
USP
7 (catalytic domain aa 213-548) (Accession Nr. Q93009) fused to a N-terminal His-tag.



Crossreactivity

Human



Formulation

Liquid. In HEPES, NaCl, DTT and Glycerol.



Other Product Data


Use:
Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 1-5nM. 15 minute pre-incubation with 10mM DTT is recommended to achieve maximum activity.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin Specific Peptidase 7 (
USP
7), also known as Herpes Virus-associated ubiquitin Specific Protease (HA
USP
), is a widely expressed deubiquitinating enzyme belonging to the peptidase C19 family. It has a predicted molecular weight of 130 kDa. Human
USP
7 is 1102 amino acids (aa) in length and shares 99% aa sequence identity with the mouse and rat orthologs.
USP
7 consists of a cysteine peptidase core (aa 208-560) that is flanked by an N-terminal TRAF-like domain (aa 50-205) and two C-terminal protease-resistant domains (aa 622-801 and 885-1061).
USP
7 can be phosphorylated at Ser18 and Ser963 and ubiquitinated at Lys869.
USP
7 was initially identified as a p53-interacting protein that deubiquitinates p53, thereby st
ABI
lizing the protein and inducing p53-dependent cell growth arrest and cancer.
USP
7 also targets the p53 regulatory proteins MDM2, MDMX, and Daxx, the epigenetic regulator histone 2B, and the transcription factor FoxO4. Additionally,
USP
7 interacts with the HSV-1 immediate early protein ICP0, contributing to the st
ABI
lization and transactivation cap
ABI
lity of ICP0 during HSV-1 infection.