商品编号


E-OGLYEF



品牌


Megazyme



公司


Megazyme



公司分类


Enzymes



Size

100 mUnits

商品信息

High purity recombinant
endo
-α-
N
-Acetylgalactosaminidase (
Enterococcus faecalis
) for use in research, biochemical enzyme assays and
in vitro
diagnostic analysis.?

EC 3.2.1.97
CAZy Family: GH101
CAS: 59793-96-3

endo
-alpha-
N
-acetylgalactosaminidase; glycopeptide-D-galactosyl-N-acetyl-alpha-D-galactosamine D-galactosyl-N-acetyl-galactosaminohydrolase

Recombinant.
From
Enterococcus faecalis
.
In 20 mM Tris.HCl pH 7.5, 50 mM NaCl, 5 mM EDTA.
Supplied at ~ 1 U/mL.?

Specific activity:

~ 2 U/mg (37
o
C, pH 7.5 on Gal-β-1,3-GalNAc-α-
p
NP).

St
ABI
lity:
> 2 years at -20
o
C.?

Custom quantities and formulations available up on request.?







DESCRIPTION

endo
-α-
N
-Acetylgalactosaminidase (
Enterococcus faecalis
)

EC 3.2.1.97
CAZy Family: GH101
CAS: 59793-96-3

Synonyms:
endo
-alpha-
N
-acetylgalactosaminidase; glycopeptide-D-galactosyl-N-acetyl-alpha-D-galactosamine D-galactosyl-N-acetyl-galactosaminohydrolase

Form:
In 20 mM Tris.HCl pH 7.5, 50 mM NaCl, 5 mM EDTA.

St
ABI
lity
:
?
> 2 years at -20
o
C.

Specific activity
:
~ 2 U/mg (37
o
C, pH 7.5 on Gal-β-1,3-GalNAc-α-
p
NP).

Unit definition:
One Unit of
endo
-α-
N
-acetylgalactosaminidase activity is defined as the amount of enzyme required to release one ?mole of
p
-nitrophenol per minute from Gal-β1,3-GalNAc-α-
p
NP (0.2 mM) in Tris.HCl buffer (100 mM) pH 7.5 at 37
o
C.

Specificity:
Hydrolysis of the
O
-glycosidic-α-linkage between Gal-β-1,3-GalNAc (Core 1) or GlcNAc-β-1,3GalNAc (Core 3) and a serine or threonine residue of glycopeptides and glycoproteins.

Applications:
Applications in clinical chemistry, glyco
BIOLOG
y and carbohydrate research.