商品编号


E-TSAGS



品牌


Megazyme



公司


Megazyme



公司分类


Enzymes



Size

3,000 Units at 40
o
C;
~ 6,000 Units at 60
o
C

商品信息

High purity recombinant α-Glucosidase (
Bacillus stearo
Thermo
philus
) for use in research, biochemical enzyme assays and
in vitro
diagnostic analysis.

EC 3.2.1.20
CAZy Family: GH13
CAS:
9001-42-7

alpha-glucosidase; alpha-D-glucoside glucohydrolase

Recombinant.
From
Bacillus stearo
Thermo
philus
.
In 3.2 M ammonium sulphate.
Supplied at ~ 1,500 U/mL.?

Specific activity:

~ 280 U/mg (60
o
C, pH 6.5 on
p
-nitrophenyl-α-D-glucopyranoside);
~ 135 U/mg (40
o
C, pH 6.5 on
p
-nitrophenyl-α-D-glucopyranoside).

St
ABI
lity:
> 4 years at 4
o
C.



Highly hydrolytic reuteransucrase from probiotic
Lactobacillus reuteri
strain
ATCC
55730.

Kralj, S., Stripling, E., Sanders, P., van Geel-Schutten, G. H. & Dijkhuizen, L. (2005).
Applied and Environmental Micro
BIOLOG
y
, 71(7), 3942-3950.


Link to Article

Read Abstract


Lactobacillus reuteri strain

ATCC
55730 (LB BIO) was isolated as a pure culture from a Reuteri tablet purchased from the BioGaia company. This probiotic strain produces a soluble glucan (reuteran), in which the majority of the linkages are of the α-(1→4) glucosidic type (~70%). This reuteran also contains α-(1→6)-linked glucosyl units and 4,6-disubstituted α-glucosyl units at the branching points. The LB BIO glucansucrase gene (
gtfO
) was cloned and expressed in
Escherichia coli
, and the GTFO enzyme was purified. The recombinant GTFO enzyme and the LB BIO culture supernatants synthesized identical glucan polymers with respect to linkage type and size distribution. GTFO thus is a reuteransucrase, respons
IBL
e for synthesis of this reuteran polymer in LB BIO. The preference of GTFO for synthesizing α-(1→4) linkages is also evident from the oligosaccharides produced from sucrose with different acceptor substrates, e.g., isopanose from isomaltose. GTFO has a relatively high hydrolysis/transferase activity ratio. Complete conversion of 100 mM sucrose by GTFO nevertheless yielded large amounts of reuteran, although more than 50% of sucrose was converted into glucose. This is only the second example of the isolation and characterization of a reuteransucrase and its reuteran product, both found in different
L. reuteri
strains. GTFO synthesizes a reuteran with the highest amount of α-(1→4) linkages reported to date.









DESCRIPTION

α
-
Glucosidase (
Bacillus stearo
Thermo
philus
)

EC 3.2.1.20
CAZy Family: GH13
CAS:
?9001-42-7

Synonyms:
alpha-glucosidase; alpha-D-glucoside glucohydrolase

Form:
In 3.2 M ammonium sulphate.

St
ABI
lity
:
?
> 4 years at 4
o
C.

Specific activity
:
~ 280 U/mg (60
o
C, pH 6.5 on?
p
-nitrophenyl-α-D-glucopyranoside);
~ 135 U/mg (40
o
C, pH 6.5 on?
p
-nitrophenyl-α-D-glucopyranoside).

Unit definition:
One Unit of α-D-glucosidase activity is defined as the amount of enzyme required to release one ?mole of
p
-nitrophenol per minute from 4-nitrophenyl α-D-glucopyranoside (5 mM), in sodium phosphate buffer (100 mM), pH 6.5 at 40
o
C.

Specificity:
Hydrolysis of terminal, non-reducing α-1,4-linked D-glucose residues with release of D-glucose.

Applications:
Applications in carbohydrate and biofuels research and diagnostic and analytical procedures.