商品编号


E-AMANTM



品牌


Megazyme



公司


Megazyme



公司分类


Enzymes



Size

5 Units at 90
o
C

商品信息

High purity α-Mannosidase (
Thermo
toga maritima
)

for use in research, biochemical enzyme assays and
in vitro
diagnostic analysis.

EC 3.2.1.24
CAZy Family: GH38
CAS: 9025-42-7

alpha-mannosidase; alpha-D-Mannoside mannohydrolase, alpha-D-mannopyranosidase

Recombinant.
From
Thermo
toga maritima
.
In 3.2 M ammonium sulphate.
Supplied at ~ 71 U/mL.?

Specific activity
:

4.5 U/mg protein (on
p
NP-α-D-mannopyranoside) at pH 6.0 and 90
o
C;
~ 0.2 U/mg at 40
o
C.

St
ABI
lity
:
> 4 years 4
o
C.







DESCRIPTION

α
-
(1-2,3,4,6)-D-
Mannosidase

(
Thermo
toga maritima
)

EC 3.2.1.24
CAZy Family: GH38
CAS: 9025-42-7

Synonyms:
alpha-mannosidase; alpha-D-Mannoside mannohydrolase, alpha-D-mannopyranosidase

Form:
In 3.2 M ammonium sulphate.

St
ABI
lity:
> 4 years 4
o
C.

Specific activity:
4.5 U/mg protein (on
p
NP-α-D-mannopyranoside) at pH 6.0 and 90
o
C;
~ 0.2 U/mg at 40
o
C.

Unit definition:
One Unit of α-D-Mannosidase activity is defined as the amount of enzyme required to release one ?mole of
p
-nitrophenol (
p
NP) per minute from
p
-nitrophenyl-α-D-mannopyranoside (2 mM) in MES buffer (100 mM) and CoCl
2
(1 mM) at pH 6.0 at the temperatures indicated.

Specificity:
Broad specificity; hydrolysis of terminal non-reducing alpha-D-mannose in alpha-D-mannosides.

Applications:
For use in glyco
BIOLOG
y research.