商品编号


E-SIALST



品牌


Megazyme



公司


Megazyme



公司分类


Enzymes



Size

250 Units

商品信息

High purity recombinant
exo
-α-sialidase (
Salmonella typhimurium
) for use in research, biochemical enzyme assays and
in vitro
diagnostic analysis.

EC 3.2.1.18
CAZy Family: GH33
CAS:
9001-67-6

exo
-alpha-sialidase; acetylneuraminyl hydrolase

Recombinant.
From
Salmonella typhimurium
.
In 3.2 M ammonium sulphate.
Supplied at ~ 2,500 U/mL.?

Specific activity:
?
~ 750 U/mg (37
o
C, pH 7.0 on
p
NP-α-D-
N
-acetylneuraminic acid).

St
ABI
lity
:
>2 years at 4
o
C.

Custom quantities and formulations available up on request.?







DESCRIPTION

exo
-α-Sialidase (
Salmonella typhimurium
)

EC?
3.2.1.18
CAZy Family: GH
33
CAS:?
9001-67-6

Synonyms:
exo
-alpha-sialidase; acetylneuraminyl hydrolase

Form:
In 3.2 M ammonium sulphate.

St
ABI
lity
:
?
> 2 years at 4
o
C.

Specific activity
:
~ 750 U/mg (37
o
C, pH 7.0 on
p
NP-α-D-
N
-acetylneuraminic acid).

Unit definition:
One Unit of sialidase activity is defined as the amount of enzyme required to release one ?mole of
p
-nitrophenol per minute from
p
NP-α-D-N-acetylneuraminic acid (1 mM) in sodium phosphate buffer (100 mM), pH 7.0 at 37
o
C.

Specificity:
Hydrolysis of unbranched, non-reducing terminal α-2,3-linked >> α-2,6-linked >> α-2,8-linked N-acetylneuraminic acid (NANA; Neu5Ac) residues from glycoproteins and oligosaccharides of glycoconjugates.

Applications:
Applications in clinical chemistry, glyco
BIOLOG
y and carbohydrate research.