商品编号


L0110S



品牌


Accelagen



公司


Accelagen



公司分类


Enzyme Toolbox



Concentration

135


Size

100 reactions

商品信息


Description:
TurboLigation
TM
Kit enables quick ligation of both blunt-end or cohesive-end DNA fragments in just 5 minutes at room temperature. The kit combines TurboLigase
TM
, an ultra-pure recombinant T4 DNA Ligase, and 5X TurboLigation Buffer, an optimized quick ligation buffer. A 5 minute TurboLigation reaction offers the same efficiency as a tr
ADI
tional 16
o
C overnight ligation reaction. The reaction product is ready for bacterial transformation without any further treatment.

Applications:


Adding linkers or adapters to DNA

Blunt or cohesive end ligation of duplex DNA

DNA cloning


Components:
TurboLigase
TM
: 5 unit/?l in Storage Buffer of 10 mM Tris-HCl, pH 7.5, 50 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, and 50% glycerol
5X TurboLigation Buffer: 250 mM Tris-HCl, pH 7.5, 50 mM MgCl
2
, 5 mM dithiothreitol, 5 mM ATP, 25% polyethylene glycol 8000


St
ABI
lity and Storage:
TurboLigase and 5X TurboLigation Buffer are stable at -20
o
C for 12 months from date of receipt.
Store TurboLigase and 5X TurboLigation Buffer at -20
o
C.
Shipped in gel packs.