商品编号


40415



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Kinase(s)



Size

10 ?g

商品信息





Species: Human

Host Species: Sf9 insect cells

MW: 33 kDa





Genbank #: NM_00731

Tag(s): N-terminal His-tag

a.a: 229–512





Description:
Human Abelson murine leukemia viral oncogene homolog 1 (Abl-1), GenBank Accession No. NM_005157, a.a. 229-512, with Thr-to-Ile mutation on a.a. 315* and N-terminal His-tag, MW = 33 kDa, expressed in a baculovirus-infected Sf9 cell expression system. *This mutation is often cited as T344I in variant B (GenBank Accession No. NM_007313).




UniProt
P00519




Synonym(s):
Abl-1(T315I), Abl-1(T344I), ABL1, Abelson murine leukemia viral oncogene homolog 1, JTK7




Specific Activity:
222 pmoles/min/μg.




Assay Conditions:
Activity was determined using a 100% phosphorylated Tyr02 peptide and a Z-lyte kinase assay (
Invitrogen
). Reaction was incubated at 30°C for 1 hour.




Formulation:
25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol, and 3 mM DTT.




Format:
Aqueous buffer solution




Storage / St
ABI
lity:
>6 months at –80°C.




Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.




Reference(s):
1. Cao, C., et al. 2003. J. Biol. Chem. 278(15): 12961–7.
2. Yano, H., et al. 2000. J. Neurosci. Res. 59(3): 356–364.




Notes:
This mutation is also cited as T344I in variant B (GenBank Accession No. NM_007313).




Warning(s):
Avoid freeze/thaw cycles




Scientific Category:
Kinase/Receptor Tyrosine




Product Type:
Recombinant Protein




Data shown is lot-specific.
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