商品编号


40411



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Kinase(s)



Size

10 ?g

商品信息





Species: Human

Host Species: Sf9 insect cells

MW: 33 kDa





Genbank #: NM_00731

Tag(s): N-terminal His-tag

a.a: 248–531





Description:
Human Abelson murine leukemia viral oncogene homolog 1 (Abl-1) (GenBank Accession No. NM_007313), a.a. 248-531, with N-terminal His-tag, MW = 33 kDa, expressed in a Baculovirus-infected Sf9 cell expression system




UniProt
P00519




Synonym(s):
ABL1, ABL-1, Abelson murine leukemia viral oncogene homolog 1, JTK7




Specific Activity:
2,700 pmoles/min/μg.




Assay Conditions:
Assay was performed in Kinase buffer containing 0.2 mM DTT using Abltide substrate (0.2 mg/ml) and 20 mM ATP. Reaction was done at 30°C for 30 min. Amount of ATP transferred was calculated using Kinase-Glo
?
reagent (
Promega
).




Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, 200 mM imidazole, and 20% glycerol.




Format:
Aqueous buffer solution




Storage / St
ABI
lity:
>6 months at –80°C.




Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.




Reference(s):

1. Cao, C., et al. 2003. J. Biol. Chem. 278(15):12961–7.
2. Yano, H., et al. 2000. J. Neurosci. Res. 59(3):356–364.

Application Reference:

LASP1 is a novel BCR-ABL substrate and a phosphorylation-dependent binding partner of CRKL in chronic myeloid leukemia
(2014)




Warning(s):
Avoid freeze/thaw cycles




Scientific Category:
Kinase/Receptor Tyrosine




Product Type:
Recombinant Protein




Data shown is lot-specific.
Contact us
for specific information on other lots.