商品编号


40613



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Kinase(s)



Size

10 ?g

商品信息





Species: Mouse

Host Species: Sf9 insect cells

MW: 47.1 kDa





Genbank #: AF415213

Tag(s): N-terminal His-tag

a.a: full length





Description:
Mouse Sphingosine kinase 1 (GenBank Accession No. AF415213), full length with N-terminal His-tag, MW=47.1 kDa, expressed in a Baculovirus infected Sf9 cell expression system.




Synonym(s):
SphK1, sphingosine kinase 1, SPK1




Specific Activity:
20 U/?g




Unit Definition:
A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P per minute at 37°C.




Assay Conditions:
Sphingosine kinase activity was determined using D-erythro-sphingosine and ATP as substrates. Assay condition: 50 mM HEPES, pH 7.4, 150 mM NaCl, 5 mM MgCl
2
, 1 mM DTT, 3 ?M Na orthovanadate, 0.5 mM ATP, 4 ?M D-erythrosphingosine, and 0.1-0.5 ?g/ml sphingosine kinase 1 at 37°C for 30 min. ATP reduction is detected using Kinase-Glo
?
Luminescent Kinase Assay Platform (
Promega
Corporation, M
ADI
son, WI).




Formulation:
25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.




Format:
Aqueous buffer solution




Storage / St
ABI
lity:
>6 months at –80°C.




Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.




Reference(s):
1. Delon, C., et al., J. Biol. Chem 279 (43) 44763-44774 (2004)
2. Taha, TA et al., FASEB J. 20(3):482-4 (2005)




Warning(s):
Avoid freeze/thaw cycles




Scientific Category:
Kinase/Lipids




Product Type:
Recombinant Protein




Data shown is lot-specific.
Contact us
for specific information on other lots.