商品编号


J4210



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


Proteasomes / Activators / Subunits



Size

1 Kit

商品信息

N/A

Additional Information






Product Name:

Proteasome Inhibitor Screening Kit I



Also Known As:

N/A



Catalog No.:

J4210



Size:

1 Kit



Molecular Weight:

N/A



Species:

N/A



Source:

N/A



Stock:

Enzyme stocked in 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol; Substrate stocked in DMSO



Concentration:

See tube label



Quality Assurance:

Enzyme >95% by SDS-PAGE; Substrate > 98% by HPLC and NMR



Storage:

Store at -80°C; avoid multiple freeze-thaw cycles



PDF Data Sheet:

PDF datasheet
,
MSD
S



NCBI RefSeq:

N/A



Image(s):

N/A



Shipping Method:

Dry ice shipping



References:

N/A




Details

* Each of the supplied fluorogenic substrate is sufficient for 165 x 150 ?l reactions. The supplied 20S proteasome is sufficient for 53 X 150 ?l reactions.

Description
Proteasome Inhibitor Screening Kit I is designed for assaying the proteasome’s chymotrypsin-like, trypsin-like and caspase-like activities in vitro using highly purified bovine 20S proteasome. The proteasome cleaves the supplied fluorogenic substrates and the released AMC fluorescence can be monitored using a plate reader or fluorometer at the excitation/emission wavelength of 360nm/460nm, respectively.
AMC is used to produce a standard curve using a concentration range of 10-100 pmol.
A typical 150 ?l reaction contains 10 nM bovine 20S proteasome, 50 ?M fluorogenic substrate in the Proteasome Assay Buffer.

Components
? AMC (0.1mM in DMSO)........................................................30 ?l ? 1000X SUC-LLVY-AMC (50 mM in DMSO)...........................25 ?l
? 1000X BOC-LRR-AMC (50 mM in DMSO)...........................25 ?l ? 1000X Z-LLE-AMC (50 mM in DMSO)..................................25 ?l
? 40X Bovine 20S Proteasome (400 nM)..............................200 ?l ? 20X Proteasome Assay Buffer...........................................1.2 ml