商品编号


B1300



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


E1 Activating Enzymes



Size

25 ?g


商品信息

SAE1/SAE2 is the heterodimeric E1 enzyme that conjugates SUMO to protein substrates. The conjugation process is very similar to that of protein ubiquitination. SUMO is conjugated onto the catalytic cysteine residue of SAE2 by formation of a thioester bond in an ATP-dependent reaction. The activated SUMO is then passed on to an E2 (Ubc9) to be finally attached to a substrate.

Additional Information






Product Name:

6xHis-SAE1/SAE2



Also Known As:

SAE1 (AOS1; SUA1; UBLE1A; HSPC140; FLJ3091); SAE2 (RBBP8; RIM; CTIP; JWDS; SCKL2)



Catalog No.:

B1300



Size:

25 ?g



Molecular Weight:

SAE1 (33 kDa), SAE2 (71.2 kDa)



Species:

Human



Source:

Bacterial recombinant



Stock:

20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol



Concentration:

See tube label



Quality Assurance:

~95% by SDS-PAGE, see datasheet for gel image



Storage:

Store at -80°C; avoid multiple freeze-thaw cycles



PDF Data Sheet:

PDF Datasheet
，
MSD
S



NCBI RefSeq:

NM_005500 (SAE1); NM_002894 (SAE2)



Image(s):

(Click image to enlarge)








Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s
Lane 2: 5 ?g purified 6xHis-SAE1/SAE2







Shipping Method:

Dry ice shipping



References:

1. Desterro JM,
et al
. (1999) J Biol Chem 274(15), 10618 – 10624.
2. Melchior F (2000) Annu Rev Cell Dev Biol 16, 591 – 626. 3. Schulman BA,
et al
. (2009) Nat Rev Mol Biol 10(5), 319 – 331.




Details

SAE1/SAE2 is the heterodimeric E1 enzyme that conjugates SUMO to protein substrates. The conjugation process is very similar to that of protein ubiquitination. SUMO is conjugated onto the catalytic cysteine residue of SAE2 by formation of a thioester bond in an ATP-dependent reaction. The activated SUMO is then passed on to an E2 (Ubc9) to be finally attached to a substrate.

Images:
(Click image to enlarge)








Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s
Lane 2: 5 ?g purified 6xHis-SAE1/SAE2