商品编号


B1400



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


E1 Activating Enzymes



Size

25 ?g


商品信息

APPBP1/UBA3 is the heterodimeric E1 enzyme that conjugates Nedd8 to protein substrates. Nedd8 is conjugated onto the catalytic cysteine residue of UBA3 by formation of a thioester bond in an ATP-dependent reaction. The activated Nedd8 is then passed on to an E2 (Ubc12) to be finally attached to a substrate. Nedd8 is mainly conjugated on Cullins to increase the activity of the Cullin – RING E3 Ub ligases.

Additional Information






Product Name:

APPBP1/6xHis-UBA3



Also Known As:

APPBP1 (NAE1;HPP1; ula-1; A-116A10.1); UBA3 (UBE1C; hUBA3; MGC22384; DKFZp566J164)



Catalog No.:

B1400



Size:

25 ?g



Molecular Weight:

APPBP1 (60.2 kDa), UBA (51.8 kDa)



Species:

Human



Source:

Bacterial recombinant



Stock:

20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol



Concentration:

See tube label



Quality Assurance:

~95% by SDS-PAGE, see datasheet for gel image



Storage:

Store at -80°C; avoid multiple freeze-thaw cycles



PDF Data Sheet:

PDF Datasheet
，
MSD
S



NCBI RefSeq:

NM_003905 (APPBP1); NM_003968 (UBA3)



Image(s):

(Click image to enlarge)








Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s
Lane 2: 5 ?g purified APPBP1/6xHis-UBA3







Shipping Method:

Dry ice shipping



References:

1. Whitby FG,
et al
. (1998) J Biol Chem 273(52), 34983 – 34991.
2. Osaka F,
et al
. (1998) Genes Dev 12(15), 2263 – 2268. 3. Walden H,
et al
. (2003) Mol Cell 12(6), 1427 – 1437.
4. Schulman BA,
et al
. (2009) Nat Rev Mol Biol 10(5), 319 – 331.




Details

APPBP1/UBA3 is the heterodimeric E1 enzyme that conjugates Nedd8 to protein substrates. Nedd8 is conjugated onto the catalytic cysteine residue of UBA3 by formation of a thioester bond in an ATP-dependent reaction. The activated Nedd8 is then passed on to an E2 (Ubc12) to be finally attached to a substrate. Nedd8 is mainly conjugated on Cullins to increase the activity of the Cullin – RING E3 Ub ligases.

Images:
(Click image to enlarge)








Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s
Lane 2: 5 ?g purified APPBP1/6xHis-UBA3