商品编号


C1200



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


E2 Conjugating Enzymes



Size

25 ?g


商品信息

Ube2B is an E2 enzyme, which is part of the E1, E2, and E3 cascade respons
IBL
e for ubiquitination of protein substrates. It shares 95% amino acid sequence identity with Ube2A. One function of Ube2B involves the formation of Ube2B/ Rad18 (an E3 enzyme) complex to monoubiquitinate proliferating cell nuclear antigen (PCNA) thus activating it to repair DNA damage. The Ube2B/Rad18 complex is recruited to the replication fork of the damaged DNA to start the post-replication repair.

Additional Information






Product Name:

6xHis-Ube2B



Also Known As:

HR6B; UBC2; HHR6B; RAD6B; E2-17kDa



Catalog No.:

C1200



Size:

25 ?g



Molecular Weight:

17.3 kDa



Species:

Human



Source:

Bacterial recombinant



Stock:

20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol



Concentration:

See tube label



Quality Assurance:

~95% by SDS-PAGE, see datasheet for gel image



Storage:

Store at -80°C; avoid multiple freeze-thaw cycles



PDF Data Sheet:

PDF Datasheet
，
MSD
S



NCBI RefSeq:

NM_003337



Image(s):

(Click image to enlarge)








Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s
Lane 2: 5 ?g purified 6xHis-Ube2B







Shipping Method:

Dry ice shipping



References:

1. Jentsch S,
et al
. (1987) Nature 329(6135), 131 – 134.
2. Koken MH,
et al
. (1991) Proc Natl Acad Sci USA 88(20), 8865 – 8869. 3. Bailly V,
et al
. (1994) Genes Dev 8(7), 811 – 820.
4. Tsuji Y,
et al
. (2008) Genes Cells 13, 343 – 354.




Details

Ube2B is an E2 enzyme, which is part of the E1, E2, and E3 cascade respons
IBL
e for ubiquitination of protein substrates. It shares 95% amino acid sequence identity with Ube2A. One function of Ube2B involves the formation of Ube2B/ Rad18 (an E3 enzyme) complex to monoubiquitinate proliferating cell nuclear antigen (PCNA) thus activating it to repair DNA damage. The Ube2B/Rad18 complex is recruited to the replication fork of the damaged DNA to start the post-replication repair.

Images:
(Click image to enlarge)








Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s
Lane 2: 5 ?g purified 6xHis-Ube2B