商品编号


C2000



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


E2 Conjugating Enzymes



Size

25 ?g


商品信息

Ube2E3 (also known as UbcH9) is an E2 enzyme, which is part of the E1, E2, E3 cascade that is respons
IBL
e for ubiquitination of protein substrates. It has been found to interact with the E3 enzyme Nedd4-2 in order to regulate ubiquitination of the epithelial Na+ channel (ENaC). Studies have shown that Ube2E3 may be able to associate with the substrate directly, which implies the assistance of an E3 may not be required under all circumstances.

Additional Information






Product Name:

6xHis-Ube2E3 (UbcH9)



Also Known As:

UBCH9; UbcM2



Catalog No.:

C2000



Size:

25 ?g



Molecular Weight:

22.9 kDa



Species:

Human



Source:

Bacterial recombinant



Stock:

20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol



Concentration:

See tube label



Quality Assurance:

~95% by SDS-PAGE, see datasheet for gel image



Storage:

Store at -80°C; avoid multiple freeze-thaw cycles



PDF Data Sheet:

PDF Datasheet
，
MSD
S



NCBI RefSeq:

NM_006357



Image(s):

(Click image to enlarge)








Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s
Lane 2: 5 ?g purified 6xHis-Ube2E3 (UbcH9)







Shipping Method:

Dry ice shipping



References:

1. Ito K,
et al
. (1999) Cyotgenet Cell Genet 84 (1 – 2), 99 – 104.
2. Ito K,
et al
. (2001) Eur J Biochem 268, 2725–2732. 3. Debonneville C,
et al
. (2004) Mol Cell Biol 24, 2397–2409.
4. Plafker KS,
et al
. (2008) Invest Ophthalmol Vis Sci 49(12), 5611 – 5618.




Details

Ube2E3 (also known as UbcH9) is an E2 enzyme, which is part of the E1, E2, E3 cascade that is respons
IBL
e for ubiquitination of protein substrates. It has been found to interact with the E3 enzyme Nedd4-2 in order to regulate ubiquitination of the epithelial Na+ channel (ENaC). Studies have shown that Ube2E3 may be able to associate with the substrate directly, which implies the assistance of an E3 may not be required under all circumstances.

Images:
(Click image to enlarge)








Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s
Lane 2: 5 ?g purified 6xHis-Ube2E3 (UbcH9)