商品编号


C2500



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


E2 Conjugating Enzymes



Size

25 ?g


商品信息

Ube2I (Ubc9) is an E2 enzyme in the E1, E2, and E3 cascade to link SUMO to a substrate. This E2 shares a similar sequence and structure to the Ub E2s and is the only known SUMO conjugating enzyme at this time. Ube2I shares 56% amino acid identity with the yeast homolog called Ub – conjugating enzyme 9. While a hypothesis has been proposed that Ube2I may not need an E3 to ligate SUMO.

Additional Information






Product Name:

6xHis-Ube2I



Also Known As:

P18; UBC9; C358B7.1



Catalog No.:

C2500



Size:

25 ?g



Molecular Weight:

18 kDa



Species:

Human



Source:

Bacterial recombinant



Stock:

20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol



Concentration:

See tube label



Quality Assurance:

~95% by SDS-PAGE, see datasheet for gel image



Storage:

Store at -80°C; avoid multiple freeze-thaw cycles



PDF Data Sheet:

PDF Datasheet
，
MSD
S



NCBI RefSeq:

NM_003345



Image(s):

(Click image to enlarge)








Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s
Lane 2: 5 ?g purified 6xHis-Ube2I







Shipping Method:

Dry ice shipping



References:

1. Wang ZY,
et al
. (1996) J Biol Chem 271(40), 24811 – 24816.
2. Golebiowski F,
et al
. (2004) Arch Biochem Biophys 428(2), 160 – 164. 3. Johnson ES, (2004) Rev. Biochem. 73:355–382.
4. Reverter D,
et al
. (2005) Nature 435(7042), 687 – 692.




Details

Ube2I (Ubc9) is an E2 enzyme in the E1, E2, and E3 cascade to link SUMO to a substrate. This E2 shares a similar sequence and structure to the Ub E2s and is the only known SUMO conjugating enzyme at this time. Ube2I shares 56% amino acid identity with the yeast homolog called Ub – conjugating enzyme 9. While a hypothesis has been proposed that Ube2I may not need an E3 to ligate SUMO.

Images:
(Click image to enlarge)








Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s
Lane 2: 5 ?g purified 6xHis-Ube2I