商品编号


C2601



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


E2 Conjugating Enzymes



Size

100 ?g


商品信息

UbE2J1 is an E2 enzyme, which is part of the E1, E2, and E3 cascade respons
IBL
e for ubiquitination of protein substrates. UBE2J1 is a membrane protein localized on the endoplasmic reticulum (ER), where it catalyzes ubiquitination of misfolded membrane proteins with other ubiquitination components. UbE2J1(1-282) lacks the C-terminal membrane-localization region, but still possesses Ub conjugating activity.

Additional Information






Product Name:

6xHis-Ube2J1(1-282)



Also Known As:

UBC6; UBC6E; Ubc6p; CGI-76; NCUBE1; HSPC153; HSPC205; NCUBE-1; HSU93243



Catalog No.:

C2601



Size:

100 ?g



Molecular Weight:

31.2 kDa



Species:

Human



Source:

Bacterial recombinant



Stock:

20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol



Concentration:

See tube label



Quality Assurance:

~80% by SDS-PAGE, see datasheet for gel image



Storage:

Store at -80°C; avoid multiple freeze-thaw cycles



PDF Data Sheet:

PDF Datasheet
，
MSD
S



NCBI RefSeq:

N/A



Image(s):

(Click image to enlarge)








Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s
Lane 2: 5 ?g purified 6xHis-Ube2J1(1-282)







Shipping Method:

Dry ice shipping



References:

1. Lester D,
et al
. (2000) Biochem Biophys Res Commun 269, 474 – 480.
2. Tiwari S,
et al
. (2001) J Biol Chem 276, 16193 – 16200. 3. Lenk U,
et al
. (2002) J Cell Sci 115(Pt 14), 3007 – 3014.
4. Wang X,
et al
. (2009) J Cell Biol 187(5), 655 – 668.




Details

UbE2J1 is an E2 enzyme, which is part of the E1, E2, and E3 cascade respons
IBL
e for ubiquitination of protein substrates. UBE2J1 is a membrane protein localized on the endoplasmic reticulum (ER), where it catalyzes ubiquitination of misfolded membrane proteins with other ubiquitination components. UbE2J1(1-282) lacks the C-terminal membrane-localization region, but still possesses Ub conjugating activity.

Images:
(Click image to enlarge)








Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s
Lane 2: 5 ?g purified 6xHis-Ube2J1(1-282)