商品编号


TG1035



品牌


TopoGEN



公司


TopoGEN



公司分类


Antibody kits



Size

50 Analyses

商品信息

The immunoprecpitation (IP) kit contains the key reagents and protocol for immunoprecipitating p170 human topoisomerase II.? The method should work well with other cells besides humans.? For example, mouse, rat and monkey cells will cross react with

topoisomerase II antibody.? The antibody is specific for p170 (topo II alpha) in human (HeLa) cells and although we presume it will behave similarly in other species with cross reactive topo II isozymes, this should be carefully evaluated.


Kit Contents:


Anti-topo II (p170) Antibody, (TG2011-1). This is a rabbit antibody (polyclonal) directed against the C-Terminal region of p170. A peptide was used to raise this antiserum; the antibody has been immunoaffinity purified. The antibody is specific for topo II alpha (p170) form. Kit contains 100 ul of antibody.

Human topo II p170
Marker
. The
Marker
is ready to load in 1X SDS PAGE Lo
ADI
ng buffer. Lo
ADI
ng 8-10 ul of this
Marker
will give a re
ADI
ly detectable band on commassie blue staining. Kit contains 100 ul of
Marker
.

Detailed Protocol for immunoprecipitation

Detailed Protocol for preparation of nuclear extracts