商品编号


TG4110



品牌


TopoGEN



公司


TopoGEN



公司分类


Inhibitors and poisons



Size

Lyophilized

商品信息


Camptothecin Description
Camptothecin is a topo I poison that both inhibits enzyme activity and st
ABI
lizes formation of topo I cleavage complexes (Hsiang et al J. Biol. Chem. 260:14873-14878, 1985).?
TopoGEN
provides this reagent as a control inhibitor/poison of topoisomerase I.? For in vitro cleavage detection, we recommend that the drug be tested in the range 100 uM; however, cleavages are strongly influenced by the amount of topoisomerase in the reaction as well as the type of assay being employed (SDS-K+ vs. R
ADI
oactive End Labeled DNA vs. testing for plasmid nick- ing).? Note that larger amounts of enzyme will be required to detect cleavage complex, compared to assays that measure catalytic activity.? This is because cleavage assays are stoichiometric based assays that consume topo I.


Chemical Name:
(S)-4-Ethyl-4,10-dihydroxy-1H-pyrano[3',4':6,7]indolizino-[1,2-b]quinoline-3,14(4H,12H)-dione


Molecular Formula:
C20H16N2O4


Molecular Weight:?
348.35

?


Vial Contents:
TG4110 contains lyophilized Camptothecin.? Addition of 0.25 ml of solvent to dry drug (methanol or DMSO) will yield a working stock of 10 mM.? In some cases, the drug is supplied already s
USP
ended in DMSO at 10 mM (0.25 ml) and is ready for use.? The drug is pre-tested and shown to induce topo I cleavages using purified human Topoisomerase I from
TopoGEN
, Inc.

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