商品编号


LS01440



品牌


Worthington



公司


Worthington Biochemical Corporation



公司分类


Products



Size

10 ml

商品信息
Deoxyribonucleic Acid, Denatured, FragmentedSource: Salmon TestesPrepared from purified salmon testes DNA (Code: SDNA) by mechanical shearing and heat denaturation to an average fragment size of 200-1000 base pairs. To reverse any renaturation occurring during storage this material should be briefly boiled and rapidly chilled before use. Recommended concentration for use is 100?g/ml. A solution at 5 mg/ml in 0.05M NaCl.Store at -20°C.REQUIRES SPECIAL SHIPPING: ICE PACK
Worthington
offers DNA purified from these sources:
Calf thymus
: (Code: DNA) Prepared and purified by a method developed at
Worthington
to have lower protein and RNA contamination than most other commercial preparations. This highly polymerized DNA is an excellent substrate for deoxyribonuclease. A sodium salt, it must be converted by adding magnesium ions to be suscept
IBL
e to DNase.
Calf thymus DNA, covalently bound to cellulose
is also available:
DNA Cellulose, Double-Stranded
(Code: DNACELDS)
DNA Cellulose, Single-Stranded
(Code: DNACELSS)
Salmon Testes
: (Code: SDNA) Prepared by a modification of the method of Emanuel and Chaikoff,
J. Biol. Chem., 203
, 164, (1953). A minimum of 75% native nucleic acid.
Salmon Testes DNA, Denatured & Fragmented
(Code: SDNAD) is also available. Prepared by mechanical shearing and heat denaturation.
Escherichia coli
: (Code: DNAEC) Isolated as described by Marmur,
J. Mol. Biol., 3
, 208, 1961.
Lam
BD
a phage DNA
(Code: DNAL) is prepared from CsCl purified phage and is purified to an A
260
/A
280
≥1.8. Homogeneous in agarose gel electrophoresis. A solution in 10 mM Tris-HCl pH 8.0 with 1 mM EDTA.
DNA fragments
prepared by restriction endonuclease digestion of purified lam
BD
a DNA are available (Codes: DNALBSTE; DNALECOR; DNALHIND). Supplied as solutions in 10 mM Tris-HCl pH 8.0 with 1 mM EDTA.
Technical Note
: One A
260
unit = 50 ?g DNA.
St
ABI
lity/Storage:
DNAL: Storage buffer 10 mM Tris-HCl, pH 8.0 containing 1 mM EDTA. Store at -20°C. ?Once thawed keep at 2-8°C.
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