商品编号


LS006365



品牌


Worthington



公司


Worthington Biochemical Corporation



公司分类


Products



Size

20 mg

商品信息
Elastase, PurifiedChromatographically purified. A lyophilized powder.Store at 2-8°C.REQUIRES SPECIAL SHIPPING: ICE PACK≥8 units per mg protein
Source:
Porcine Pancreas
I.U.B.: 3.4.21.36
Porcine pancreatic elastase has a molecular weight of 25.9 kDa, and a pH optimum of 8.5. ?While elastase will hydrolyze a wide variety of protein substrates, it is unique among proteases in its
ABI
lity to hydrolyze native elastin, a substrate not attacked by trypsin, chymotrypsin or pepsin. Soybean trypsin inhibitor and kallikrein inhibitor suppress proteolytic but not elastolytic activity. ?Elastase is assayed using a method adapted from that of Feinstein
et al., Biochem. Biophys. Res. Comm., 50
, 1020 (1973) and using the more soluble substrate of Bieth
et al., Biochem. Med., 11,
350 (1974).
St
ABI
lity/Storage:
Elastase is unstable at pH ≤ 3.5. When stored as a dry powder the enzyme is stable for 6-12 months at 2-8°C. ?Elastase product codes: ES and ESL have poor solubility at neutral pH and at concentrations greater than 0.25%. ?It is helpful to make primary solutions in KCl or alkaline buffers before diluting into the reaction mixtures or media, compensating for ionic strength or pH changes. ?Stable at pH 4.0-10.4.
Technical Notes
: 1 SucAla
3
NA unit is approximately equivalent to 6 elastin digestion units. Aqueous liquid s
USP
ensions should be aseptically handled to avoid bacterial contamination. Due to the viscous nature of the aqueous s
USP
ension (Code: ES) the vial should be rinsed to recover contents.
Unit Definition
: One Unit cleaves one micromole of N-succinyl-L-alanyl-L-alanyl-L-alanine-p-nitroanilide per minute at 25°C, pH 8.0.
Related Products:
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(CLS1-4/CLSPA)
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Hyaluronidase (HSE/HSEP)
Neutral Protease (Dispase, NPRO)
Papain (PAP/PAPL/PAP2)
Pepsin (PM)
Proteinase K (PROK)
Trypsin (TL/TRL/TRL3/TRLS/TRTPCK)
Trypsin Inhibitors (LBI/OI/SI/SIC)
Cell Isolation Optimizimg System (CIT)
Hepatocyte Isolation System (HIS)
Neonatal Cardiomyocyte Isolation System (NCIS)
Papain Dissociation System (PDS/PDS2)