商品编号


LS004798



品牌


Worthington



公司


Worthington Biochemical Corporation



公司分类


Products



Size

45 ku

商品信息
Nuclease, MicrococcalChromatographically purified to be essentially homogeneous chromatographically and electrophoretically (SDS-PAGE). A lyophilized powder.Store at 2-8°C.≥6,000 units per mg protein
Source:
Staphylococcus aureus (Strain
ATCC
#27735)
I.U.B.: 3.1.31.1
Micrococcal nuclease catalyzes cleavage of both DNA and RNA to yield 3'-nucleotides. It exhibits exo- and endo-5'-phosphodiesterase activities. The enzyme catalyzes preferential endohydrolysis of the RNA and DNA at sites rich in adenylate or uridylate and deoxyadenylate or thymidylate. The enzyme has a molecular weight of 16,807 daltons and is calcium dependent. The pH optimum is 9.2 but varies depending upon the concentration of ionized calcium present.
Unit Definition
: One Unit corresponds to a change in optical density of 1.0 at 260 nm at 37°C, pH 8.0, using DNA as the substrate.
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