商品编号


LS04070



品牌


Worthington



公司


Worthington Biochemical Corporation



公司分类


Products



Size

10 ku

商品信息
Nuclease, S1Source: Aspergillus oryzaeChromatographically purified. Specific for single-stranded DNA (ssDNA) degradation. Activity on native (ds) DNA undetectable under the assay conditions. A frozen solution in 30mM sodium acetate, pH 4.6, 50mM NaCl, 1mM ZnCl2, and 50% glycerol.Store at -20°C.REQUIRES SPECIAL SHIPPING: ICE PACK≥100,000 to 500,000 units per ml
Source:

Aspergillus oryzae
I.U.B.: 3.1.30.1
Nuclease S1 isolated from certain Neurospora and Aspergillus species specifically hydrolyzes both terminal and internal phosphodiester bonds of single-stranded DNA and RNA. Nuclease S1 has a molecular weight of approximately 34 kDa and exists as a monomer. The optimum pH range is 4.0-4.6, and it is activated by Zn
2+
and/or Ca
2+
. Inhibitors are EDTA, citrate and high concentrations of SDS.
St
ABI
lity/Storage:
For long term storage in solution, for up to six months, dilute NUCSI to ≥6000 u/ml in water and freeze in aliquots. Dilute solutions can be st
ABI
lized by adding 0.1% albumin (
Worthington
Code: BSANF) and 10% glycerol.
Unit Definition
: One Unit hydrolyzes one microgram of denatured calf thymus DNA per minute at 37°C, pH 4.6.
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