商品编号


LS003119



品牌


Worthington



公司


Worthington Biochemical Corporation



公司分类


Products



Size

100 mg

商品信息
Papain, LyophilizedSupplied as a lyophilized powder prepared from a two times crystalline s
USP
ension, Code: PAP. To insure full activity, the enzyme should be incubated in a solution containing 1.1mM EDTA, 0.067mM mercaptoethanol and 5.5mM cysteine-HCl for 30 minutes. It is recommended that the enzyme be 0.22 micron filtered after dissolution and prior to use.Store at 2-8°C.Activates to at least 15 units per mg protein
Source:
Carica Papaya Latex
I.U.B.: 3.4.22.2
Papain is a sulfhydryl protease from
Carica papaya
latex. It has a molecular weight of 23 kDa and an optimum pH range of 6.0-7.0. ?The action of papain on leucine methyl ester produces an insoluble polyleucine peptide. Papain breaks down the intercellular matrix of cartilage. Papain is activated by cysteine, sulfide, and sulfite. St
ABI
lizing agents are EDTA, cysteine and dimercaptoethanol.
St
ABI
lity/Storage:
Stable for 6-12 months at 2-8°C. ?Do not freeze aqueous s
USP
ensions.
Technical Notes
: Papain preparations should be incubated in the activation solution before use to ensure full activity. Applications include antibody fragmentation and primary/neural cell isolation.
Unit Definition
: One Unit hydrolyzes one micromole of benzoyl-L-arginine ethyl ester per minute at 25°C, pH 6.2, after activation in a solution containing 1.1 mM EDTA, 0.067 mM mercaptoethanol and 5.5 mM cysteine-HCl for 30 minutes.
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Cell Isolation Optimizimg System (CIT)
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