商品编号


LK003176



品牌


Worthington



公司


Worthington Biochemical Corporation



公司分类


Products



Size

1 vi

商品信息
PDS Kit, Papain VialA component of the Papain Dissociation System, for use in the tissue dissociation method of Huettner, J.E., and Baughman, R.W.: J. Neuroscience, 6, 3044 (1986). Contains papain, L-cysteine, and EDTA. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 5 ml of EBSS or equivalent yields a solution at 20 units of papain per ml in 1mM L-cysteine with 0.5mM EDTA.Store at 2-8°C.≥100 units per vial
Source:
Carica Papaya Latex
I.U.B.: 3.4.22.2
Papain is a sulfhydryl protease from
Carica papaya
latex. It has a molecular weight of 23 kDa and an optimum pH range of 6.0-7.0. ?The action of papain on leucine methyl ester produces an insoluble polyleucine peptide. Papain breaks down the intercellular matrix of cartilage. Papain is activated by cysteine, sulfide, and sulfite. St
ABI
lizing agents are EDTA, cysteine and dimercaptoethanol.
St
ABI
lity/Storage:
Stable for 6-12 months at 2-8°C. ?Do not freeze aqueous s
USP
ensions.
Technical Notes
: Papain preparations should be incubated in the activation solution before use to ensure full activity. Applications include antibody fragmentation and primary/neural cell isolation.
Unit Definition
: One Unit hydrolyzes one micromole of benzoyl-L-arginine ethyl ester per minute at 25°C, pH 6.2, after activation in a solution containing 1.1 mM EDTA, 0.067 mM mercaptoethanol and 5.5 mM cysteine-HCl for 30 minutes.
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